农杆菌介导的高粱遗传转化研究进展

Progress on Agrobacterium-Mediated Genetic Transformation in Sorghum

高粱(Sorghum bicolor(L.)Moench)对高温和干旱具有很强的耐受能力,是干旱、半干旱地区人们理想的谷类作物。通过现代遗传转化的手段提高高粱的产量,改善其品质显得尤为重要。农杆菌介导的遗传转化是将外源基因转入植物细胞最常用的方法。然而,从2000年首次报道农杆菌介导的高粱遗传转化以来,至今成功的例子仍然非常有限。优化影响农杆菌介导的高粱遗传转化的关键参数(筛选适合转化的基因型与外植体,选择合适的农杆菌株,建立有效的组织培养和再生体系,利用高效的选择和筛选系统),必将会提高转化效率,拓宽转化范围,为进一步开展高粱的遗传转化研究提供科学依据。

Sorghum(Sorghum bicolor(L.) Moench) has strong tolerance for high temperature and drought, so it is an ideal cereal crop in arid and semi-arid areas. It is very important to improve its production and quality by modern means of genetic transformation. Agrobacterium-mediated transformation is the most commonly method for transferring the foreign genes into plant cells. The first Agrobacterium-mediated transformation of sorghum has been reported in 2000, but up to now the successful examples are still very limited. Many factors influencing Agrobacterium-mediated transformation of sorghum have been investigated and elucidated, which include plant genotype and explant type, suited Agrobacterium strain, tissue culture and regeneration condition, and selecting and screening system. Optimization the key parameters of Agrobacterium-mediated transformation of sorghum will certainly improve the transformation efficiency, expand the transformation scope and provide a scientific basis for further research on genetic transformation of sorghum.