华北紫丁香肉桂酸4-羟化酶(SoC4H)基因的克隆及表达分析

Cloning and Expression Analysis of Cinnamic Acid 4-hydroxylase Gene from Syringa oblata Lindl.

华北紫丁香系木犀科丁香属落叶灌木,是北方著名的花木之一。为了进一步研究华北紫丁香肉桂酸4-羟化酶(SoC4H)在花色苷代谢途径中的表达特性,利用RT-PCR技术对SoC4H基因进行分离克隆,并对其进行生物信息学分析,同时利用实时荧光定量PCR技术分析了该基因的组织表达模式。结果表明,SoC4H基因的最大开放阅读框(opening reading frame,ORF)长为1 518 bp,编码505个氨基酸残基;该基因的基因组序列无内含子;SoC4H基因在初花期和根部的表达量较高。

Syringa oblata Lindl., as a deciduous shrub of Syringa genus in Oleaceae family, is one of famous ornamental plants to the north of China. In order to further exploring the expressional characteristics of cinnamic acid hydroxylase(C4H) of S. oblata in the anthocyanin metabolic pathway, this gene was cloned using reverse transcriptionPCR(RT-PCR), and analyzed its bioinformatics. Meanwhile we used the real time fluorescence quantitative-PCR(q RT-PCR) technology to analyse organization expression pattern of this gene. The results showed that the opening reading frame(ORF) of SoC4 H gene was 1 518 bp, and it encoded 505 amino acid residues. However, the genomic sequence of the gene had not contained introns, and the expression level of SoC4 H was more than that in early flowering stage and in roots.