甜瓜黄瓜素基因启动子的序列及元件分析

Cucumisin Gene Promoter Sequence and Element Analysis in Cucumis melon L.

本研究对甜瓜黄瓜素基因启动子进行了元件分析。讨论以采集的甜瓜叶片为试验材料,采用CTAB法提取植物DNA,并利用PCR扩增技术,成功获得了黄瓜素启动子的基因序列,利用DNAman、DNAstar等软件进行处理和分析序列结果,并采用Plant CARE和PLACE对启动子的元件进行生物信息学元件分析。分析结果表明:黄瓜素启动子与AY055805、LN713264、LN681897的同源性为100%、99%、99%。其序列含有多种高等植物果实启动子通用的顺式作用元件TATA-Box、CAAT-Box和光响应元件,同时部分序列还也参与了ABA、VP1反应和水杨酸反应。甜瓜黄瓜素启动子的研究为下一步利用该启动子进行深入的果实基因工程研究提供材料基础和理论依据。

In order to analyze the cucumisin gene promoter sequence and element in Cucumis melon L. In this study, we took the collected the melon as the test material, plant DNA was extracted by the method of CTAB and the gene sequence of the cucumisin promoter was obtained successfully by using PCR amplification. DNAman,DNAstar and other software were used to process and analyze the sequence. Besides, Plant CARE and PLACE were used to analyze bioinformatics element of promoter elements. The analysis results showed that the homologies between the cucumisin promoter and AY055805, LN713264, LN681897 were 100%, 99% and 99%,respectively. The cucumisin promoter sequences contain a variety of higher plant fruit promoter common cis-element TATA-Box, CAAT-Box and light-responsive elements. At the same time, partial sequences were also involved in ABA, VP1 reaction and salicylic acid reaction. The study on the Cucumis melon L. promoter might provide the theoretical basis for further research on the genetic engineering of fruit.