红麻S-腺苷甲硫氨酸脱羧酶基因克隆与序列分析

Cloning and Sequence Analysis of S-adenosylmethionine Decarboxylase Gene(Hc SAMDC) in Hibiscus cannabinus

多胺是植物体内参与生长发育及各种逆境胁迫响应的一类重要化合物,其合成过程受多个基因的共同调控,S-腺苷甲硫氨酸脱羧酶(S-adenosylmethionine decarboxylase,SAMDC)基因参与多胺的合成过程并发挥关键作用,为研究该基因在红麻(Hibiscus cannabinus L.)抗旱和耐盐过程中的作用,通过红麻转录组数据库筛选到SAMDC基因的核心片段,利用染色体步移技术获得了SAMDC基因c DNA全长,命名为Hc SAMDC,生物信息学分析表明:Hc SAMDC基因编码序列长1 137 bp,编码378个氨基酸,相对分子量为41.8 k D,等电点为4.86。红麻Hc SAMDC与其它植物的SAMDC同源性较高,其中与可可树氨基酸相似性为78%、与棉花SAMDC的氨基酸相似性为82%,该研究对下一步分析该基因功能、阐明麻类SAMDC基因的逆境调控机制和抗逆基因工程育种具有重要意义。

In order to study the role in the process of drought resistance and salt tolerance of S-adenosylmethionine decarboxylase in Hibiscus cannabinus. We obtained the key fragment of SAMDC gene through screening kenaf transcriptome database, SAMDC gene named HcSAMDC was isolated using chromosome walking technology. The coding sequence of HcSAMDC gene has a length of 1 137 bp, which encod 378 amino acid, molecular weight is41.8 k D, isoelectric point is 4.86, HcSAMDC gene has high similarity with other species, amino acid sequence consistency with Theobroma cacao and Gossypium raimondii is 78% and 82%, respectively. The study have great significance for analysis SAMDC gene in stress regulation mechanism and engineering breeding in hemp plant.