橡胶树HbICE1的克隆及表达模式分析

Molecular Cloning and Expression Analysis of Hb ICE1 from Hevea brasiliensis

低温是影响植物正常生长发育,导致作物产量和品质下降的主要自然灾害之一。由于橡胶树(Hevea brasiliensis)属于热带植物,不耐寒,所以极易受到低温冷害影响。而Inducer of CBF expression 1(ICE1)是低温胁迫信号通路中的重要转录激活因子,目前橡胶树冷胁迫信号途径中包括Hb ICE1在内的大部分关键基因的研究还比较匮乏。我们从橡胶树中克隆了Hb ICE1基因。经测序发现,该基因开放阅读框为1 407 bp,编码469个氨基酸,蛋白质分子质量约为51 k D,理论等电点(p I)为5.83,为亲水性蛋白。并且利用荧光定量PCR技术分析了Hb ICE1在不同激素(乙烯,脱落酸,茉莉酸)以及H2O2处理后的表达模式,结果表明乙烯、脱落酸、茉莉酸以及H2O2均能调控橡胶树Hb ICE1基因的表达,但不同胁迫处理下的表达模式存在着较大的差异,暗示着Hb ICE1在不同的激素信号途径中发挥的作用差异较大。

The plants grow well in a relatively suitable temperature conditions. Under low temperature, plants grow slowly and even show necrosis yellowing. Thus, low temperature greatly decreases plants yield. As tropical plant, rubber tree(Hevea brasiliensis) is more sensitive to low temperature and is easily impaired by chilling. Inducer of CBF expression 1(ICE1) is an important transcription factor in cold acclimation pathway. However, the cold acclimation signaling pathway is less known so far and many key regulators such as ICE1 in the rubber tree(Hb ICE1) have not been cloned. To understand the physiological response under cold stress, we isolated a Inducer of CBF expression 1(ICE1) gene from the rubber tree. This gene contained a complete open reading frame of 1 407 bp encoding a hydrophilic protein with 470 amino acids, molecular mass is about 51 k D, and the theory of isoelectric point(pI) is 5.83. Real-time quantitative PCR analysis shows that Hb ICE1 gene was mediated by ethylene(ET),abscisic acid(ABA), Jasmonate(JA) and H_2O.The expression patterns of Hb ICE1 are district under different stress treatments, indicating that Hb ICE1 plays different roles in different stress signaling pathways.