湖北省水稻区试两系不育系指纹图谱构建及遗传相似性分析

Establishment of Fingerprint and Analysis of Genetic Diversity Among Two-line Rice Sterility Lines in Regional Trials in Hubei Province

为评价2015年湖北省水稻区试两系不育系材料的遗传相似性并构建其指纹图谱,利用48个SSR分子标记对15份参试品种及6个代表性对照品种进行分析,筛选出12对扩增稳定、多态性好并分布均匀的SSR引物,共检测到47个等位基因(Na),每个标记平均检测到3.9个,每标记多态性信息含量(PIC)平均值为0.64,利用这12个引物为核心标记构建了供试材料的指纹图谱;聚类分析结果表明,15份参试品种与6个对照品种在相似系数0.66左右分为6个类群,其中6个品种与广占63-4S均归于三类群,占参试品种的40%,此外,四类群的3个品种还与三类群同属一个亚类群;其余6个品种分别与Y58S、农垦58S以及珍汕97A归于同一类群。因此,聚类分析结果表明参试品种表现出了背景来源单一、亲缘关系较近的趋势。本研究结果有助于湖北省水稻两系不育系资源利用、创新与引进,持续提高水稻育种水平。

To evaluate genetic diversity and establish fingerprint of two-line rice sterility lines in regional trials in Hubei province in 2015, 15 tested varieties and 6 typical check varieties were analyzed using 48 SSR markers. 12 markers were selected with stable amplification, rich polymorphism and evenly distribution. 47 alleles were deteted with an average of 3.9 per marker. The average value of locus polymorphism information content(PIC) was 0.64 and the fingerprinting of 21 varieties were established using 12 SSR markers with higher PIC; Cluster analysis showed that15 tested varieties and 6 check varieties were classified into 6 cluster groups with the similar coefficient of 0.66. 6varieties and Guangzhan 63-4S were classified into the 3rd group accounting for 40% of the tested varieties. Addition more, 3 varieties in the 4thgroup belonged to the same subgroup with the 3rdgroup. 6 remainders were classified into the same group with Y58 S, Nongken 58 S and Zhenshan 97 A respectively. The result of the cluster analysis indicated that tested varieties behaved single background and near relationships. This research would be helpful in selecting parents in two-line sterility lines, expanding the genetic basis of cultivated rice and improving rice yield.