莲瓣兰花朵转录组及MADS基因家族挖掘

Floral Transcriptome Sequencing and Characteristic Analysis of Cymbidium tortisepalum

莲瓣兰是我国珍贵的兰花野生资源,具有很高的观赏价值,然而其相关的分子生物学研究尚属空白。为揭示莲瓣兰花朵形成的分子机制,应用高通量测序技术对莲瓣兰花朵进行转录组分析。原始数据经过生物信息学分析后,共获得57 011条Unigene;所获得的Unigene与Nr,Swiss-prot,COG和KEGG等数据库进行搜索比对后,发现Unigene的注释总量为24 049条,约32 962条Unigene没获得注释,这些没获得注释Unigene基因可被认为是莲瓣兰新的转录本和特异基因。36条CDS基因被注释为MADS-box基因,构建系统进化树后并对31条MADS-box基因进行了聚类,其中23条为与花型发育相关的ABCDE类基因,8条为与开花时间相关的MADS基因,同时发现PI、AP3和AGL6基因表达量较高,该试验为研究兰属的花发育以及开花机理的研究提供一定的理论依据。

Cymbid ium tortisepalum belongs to a kind of precious and pretty orchid in China. However there is little known about its molecular biology. Both Illumina Hi SeqTM2000 sequencing platform and transcriptome analysis were employed to reveal the molecular mechanism of Cymbidium tortisepalum. After removing low quality sequences, Clean reads were assembled into 57 011 Unigenes. Among these unigenes, 24 049 unigene were annotated based on Nr, Swiss-prot, COG and KEGG public databases respectively. About 32 962 Unigene gene didn't get comment can be considered to be C. tortisepalum new transcripts and specific genes. 36 CDS genes encode as MADS-box gene, its transcription factors involve in flowering development based on our Illumina data set. Phylogenetic tree showed that 23 MADS genes were classified into class A, class B, class C, class D and class E subfamily, which involved in development of floral organ,and 8 MADS genes were related to blooming time.RPKM analysis indicated that PI, AP3 and AGL6 expressed much higher in flower than other MADS genes. This study provides a theoretical basis on the mechanism of flower development and blooming of Cymbidium tortisepalum.