摘要
为了开发一套能够简单快捷地筛选水稻细胞质的分子标记,本研究选用了5个配子体雄性不育系W15A、W20A、W34A、W46A和YA为材料,采用线粒体基因组DNA超纯提取和多态性扩增(random amplified polymorphic DNA,RAPD)的方法,开发了一套水稻线粒体基因组分子标记(sequence characterized amplified region,SCAR),该套分子标记共37个标记,其中16个定位水稻线粒体基因组,12个为无同源序列,其他的能在叶绿体、核以及细菌中找到同源。用这套分子标记筛选20份含红莲型不育基因orf H79的水稻材料和35个野生稻材料,均呈现出很好的细胞质多态性。因此,这些标记为水稻线粒体基因组多态性鉴定、新型不育细胞质的筛选提供一个快速的方法,同时也可用于不育系品种鉴定和水稻细胞质进化研究。
In order to develop a set of molecular markers to screen rice cytoplasmic quickly and easily, we used 5Gametophyte male sterile lines, W15 A, W20 A, W34 A, W46 A and YA, via ultra-pure mitochondrial DNA extraction and RAPD(random amplified polymorphic DNA) polymorphism amplification to develop a set of mitochondrial SCAR markers, 16 of which located at rice mitochondrial, 12 were no homologous sequences, the others likely originated from chloroplast, nuclear and bacterial. We used the molecular markers to screen 20 Rice materials with orf H79 gene and 35 wild rices, both showed good cytoplasm polymorphism. So, the set of markers provides a rapid way to identify the polymorphism of rice mitochondrial genome and screen new cytoplasm, also can be used in variety identification of cytoplasmic male sterility lines and evolution study of rice cytoplasm.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第10期2671-2679,共9页
Molecular Plant Breeding
基金
国家自然科学基金(31360332)
江西省重大科技专项(20143ACF60008)
江西省科技支撑计划(20123BBF60187)共同资助
