水稻稻瘟病抗性基因Pi25功能性SNP分子标记开发及应用

Development and Application of a Functional SNP Marker of the Blast Resistant Gene Pi25 in Rice

为了使稻瘟病抗性基因Pi25在水稻分子育种中得到高效和快速利用,本研究通过将Pi25不同等位基因测序及序列比对鉴定到一个Pi25基因内特异SNP位点。使用PCR-CTPP(PCR with confrontingtwo-pair primers)的方法加以改进开发了一套鉴定该SNP的功能性分子标记,利用该标记及待检测水稻品种基因组DNA进行PCR扩增,通过不同PCR产物条带大小将Pi25与其它等位基因区分开,可快速判断待测水稻Pi25的基因型。本方法快速简单、成本低廉,可广泛应用于水稻种质资源Pi25基因型鉴定和分子标记辅助选择育种。

To promote the application of rice blast resistance gene Pi25 in rice molecular breeding programs, a Pi25-specific SNP was identified by sequencing of Pi25 allele and up- or down-stream of them. We developed a Functional Marker based on modified PCR-CTPP(PCR with confronting two-pair primers) method. Using our SNP marker and genomic DNA of tested rice varieties, Pi25 was differentiated from other allele aided with specific PCR product by PCR amplification. So this method could be put to a good use in molecular marker-assisted selection breeding or Pi25 genotype discriminate in rice germplasm for its simplicity, speediness and low cost.