小麦分子标记在长穗偃麦草的通用性

Transferability of Molecular Markers from Wheat in Thinopyrum ponticum

利用小麦的600对SSR、391对EST-SSR、35对STS和149对PLUG引物分别对普通小麦品种YN001和十倍体长穗偃麦草基因组DNA进行扩增,旨在分析不同标记在长穗偃麦草的通用性。398(66.3%)对SSR、294(75.2%)对EST-SSR、33(94.3%)对STS和126(84.6%)对PLUG引物在长穗偃麦草有清晰的扩增条带,4种类型引物在长穗偃麦草和小麦间表现多态扩增的引物比例分别为61.0%、68.0%、82.9%和79.9%,表明这4种引物均可以用于长穗偃麦草的遗传研究,但STS和PLUG引物效果优于SSR和EST-SSR。进一步分析基因组SSR和EST-SSR引物的核心单元组成,发现二者在长穗偃麦草有效扩增的引物以二核苷酸和三核苷酸重复为主要类型。

The transferability of 600 pairs of SSR, 391 pairs of EST-SSR, 35 pairs of STS and 149 pairs of PLUG primer from wheat was tested in Thinopyrum ponticum in this study. The results showed that 398(66.3%) SSR, 294(75.2%) EST-SSR, 33(94.3%) STS and 126(84.6%) PLUG primer pairs gave evident amplification on the genomic DNA of Thinopyrum ponticum. The rates of polymorphic primers between bread wheat YN001 and Thinopyrum ponticum were 61.0%, 68.0%, 82.9% and 79.9% for the four kinds of marker, respectively. Such results indicated that SSR, EST-SSR, STS and PLUG molecular markers can be used for genetic study in Thinopyrum ponticum,while the utility of STS and PLUG markers should be superior to that of SSR and EST-SSR. Furthermore, we found that the transferable primers of SSR and EST-SSR belonged to dinucleotide or trinucleotide repeat.