梅片树组织培养中污染的抑制和愈伤组织的诱导

Study on the Pollution Abatement and Callus Induction of the Tissue Culture of Cinnammonia burmannii B1.

以富含右旋龙脑的梅片树当年生枝条作为外植体,进行梅片树组织培养技术的研究,来抑制培养中的污染,获得愈伤组织。结果表明,4月、5月、9月和10月时外植体的污染率可控制在20%以下,是进行组织培养的最佳时间;将常规消毒后的外植体接种到添加0.35‰益培隆的培养基上,培养30 d后外植体的污染率下降至23%,同时芽诱导率较高,生长状态较好;最佳的芽诱导培养基组合为MS+6-BA 2.0 mg/L+NAA0.05 mg/L,芽诱导率达89.13%,生长状态较好;小叶愈伤组织诱导的最佳培养基为:MS+6-BA 3.0 mg/L+NAA 1.0 mg/L,愈伤组织的诱导率达98.44%,生长状态良好。本研究为梅片树的组织快繁和细胞悬浮培养等的研究提供参考。

Abs tract The techniqu es of tissue culture of Cinnammonia burmannii B1. were developed from the explants taken from current-year branches of the tree with high content of d-borneol to abatement the pollution in tissue culture and obtain the callus. The results showed that the contamination rate of explants can be controlled below20% in April, May, September and October, which were the best time for tissue culture; and that explants treated by conventional disinfection were inoculated in medium with 0.35‰ Yipeilong and cultured for 30 days when the contamination rate of ecplants decreased to 23%, its launch rate was higher and buds grew better at the same time.The optimal medium for inducing shoots was MS +6-BA 2.0 mg/L+NAA 0.05 mg/L, the bud induction rate was89.13%, and the buds grew better. The optimal callus induction medium of young leaf was MS +6-BA 3.0 mg/L+NAA 1.0 mg/L, the callus induction rate was 98.44%, and the callus grew well. The study laid a solid foundation for the study of rapid propagation and cell suspension culture of Cinnamomun burmannii B1..