转杉木14-3-3基因拟南芥根系对干旱胁迫的反应

Response of Chinese Fir 14-3-3 Transgenic Arabidopsis Plants Root to Drought-Stress

为了探讨干旱胁迫对转杉木14-3-3基因拟南芥根系生长的影响,本研究以两个转杉木14-3-3基因拟南芥(OE-0和OE-4)及野生型拟南芥(Col-WT)为研究对象,利用不同甘露醇浓度(100 mmol/L,200 mmol/L,300 mmol/L和400 mmol/L)模拟干旱胁迫,并以不加甘露醇(0 mmol/L)处理为对照,检测分析不同干旱程度下三组拟南芥幼苗根长、根直径、根表面积以及根体积。结果显示,随着干旱胁迫浓度增加,Col-WT、OE-0和OE-4幼苗根长、根直径、根表面积及根体积均逐渐降低,胁迫浓度400 mmol/L时各指标均达最低值。OE-0和OE-4比Col-WT表现出更强的干旱胁迫耐受性,干旱胁迫下OE-0和OE-4幼苗根长、根直径、根表面积及根体积均高于Col-WT幼苗,并在胁迫浓度200 mmol/L时差异达最大,根长、根直径、根表面积及根体积分别比Col-WT幼苗高出119.98%、15.38%、150.94%、150.00%,185.10%、15.38%、224.52%、200.00%。OE-4比OE-0表现出更强的干旱胁迫耐受性,干旱胁迫下OE-4幼苗根长、根直径、根表面积及根体积均高于OE-0,胁迫浓度300 mmol/L时差异达最大,根长、根直径、根表面积及根体积分别比OE-0幼苗高出42.27%、7.69%、56.92%和43.47%。本研究为干旱胁迫下杉木14-3-3基因分子调控机理研究及耐旱型杉木良种繁育提供了理论参考。

In order to investigate the effects of drought stress on the root growth of Chinese fir 14-3-3 transgenic Arabidopsis, two types of Chinese fir 14-3-3 transgenic Arabidopsis(OE-0 and OE-4) and one wild-type Arabidopsis(Col-WT) were selected as the research objects. Different concentrations of mannitol(100 mmol/L, 200 mmol/L,300 mmol/L and 400 mmol/L, respectively) were to simulate drought stress, with a no-mannitol(0 mmol/L) treatment as the control group. We tested and analyzed the root length, root diameter, root surface area and root volume of three groups of Arabidopsis seedlings under different drought-stress levels. The results showed that with the increase of drought-stress concentration, the root length, root diameter, root surface area and root volume of Col-WT, OE-0 and OE-4 decreased gradually and reached their minimum under the stress concentration of400 mmol/L. The OE-0 and OE-4 groups showed stronger ability of drought-stress tolerance than Col-WT group.Specifically, root length, root diameter, root surface area and root volume of OE-0 and OE-4 were both higher than Col-WT, reaching their maximum difference under the stress concentration of 200 mmol/L, the root length,root diameter, root surface area and root volume of OE-0 and OE-4 were 119.98%, 15.38%, 150.94%, 150.00%,and 185.10%, 15.38%, 224.52%, 200.00% higher respectively than Col-WT seedlings. Compared with the OE-0group, the OE-4 group showed stronger ability of drought-stress tolerance. Specifically, root length, root diameter,root surface area and root volume of OE-4 were all higher than OE-0, and reached maximum difference under the stress of 300 mmol/L, and the root length, root diameter, root surface area and root volume of OE-4 were 42.27%,7.69%, 56.92% and 43.47% higher than OE-0 respectively. This research provided a theoretical reference for investigating the molecular-regulation mechanism of Chinese fir 14-3-3 gene under drought stress and for breeding the superior drought-resistant Chinese fir species.