水稻锌指蛋白基因OsBBX13的克隆、表达及生物信息学分析

Cloning, Expression and Bioinformatics Analysis of Zinc Finger Protein Gene OsBBX13 in Rice

锌指蛋白作为植物中重要的转录因子,在调控植物生长发育以及响应逆境过程中发挥着重要作用。水稻OsBBX13基因属于B-Box (BBX)锌指基因家族成员之一,生物信息学分析表明:OsBBX13基因启动子含有高温应答元件(HSE)、干旱应答元件(MBS)、低温应答元件(LTR)及防御应答元件(TC-rich repeats)等逆境相关元件。本研究意在挖掘逆境响应基因,探讨OsBBX13在非生物逆境中的功能,通过组织特异性荧光定量PCR研究发现,OsBBX13在叶片中表达最高,在根中表达其次,在茎、幼穗中表达较低。通过不同逆境胁迫处理后的荧光定量PCR研究发现,OsBBX13受多种逆境胁迫抑制,表达下调,表明其可能负调控响应非生物逆境。此外,本研究对基因OsBBX13进行了克隆,并对其进行了系统进化、蛋白跨膜、蛋白亚细胞定位等研究,为今后深入了解其在水稻中的生物学功能提供指导。

Zinc finger protein, an important transcription factor in plants, plays an crucial role in regulating plant growth and development, and stress responses. OsBBX13 in rice is one of the B-Box(BBX) zinc finger gene family members. Bioinformatics analysis indicates that the promoter of OsBBX13 contains stress response elements such as high temperature stress response element(HSE), drought response element(MBS), low temperature response element(LTR), and defense response element(TC-rich repeats). The purpose of this study was to clone stress response genes and explore the functions of OsBBX13 in abiotic stresses. Tissue specific fluorescence quantitative PCR showed that OsBBX13 expressed the highest level in leaves, followed by roots, and the lowest in stems and young panicles. By fluorescence quantitative PCR after different stress treatments, OsBBX13 was found to be inhibited by stresses and down-regulated, which indicated that it might be negatively regulated to abiotic stresses. In addition, the OsBBX13 gene was cloned, and its phylogenetic analysis, protein transmembrane analysis and protein subcellular localization analysis were carried out as well, which could provide guidance for further understanding of its biological functions in rice.