菠萝蜜炭疽病病原菌的形态学与多基因分子系统学鉴定

Identification of Pathogen Causing Jackfruit Anthracnose Based on Morphology and Multi-gene Sequence Analysis

为鉴定引起菠萝蜜炭疽病的病原,本研究按照常规方法对菠萝蜜炭疽病进行病原菌的分离、纯化,并用柯赫氏法则验证其致病性,结果表明分离后的病原菌可在菠萝蜜健康叶片上引起与田间炭疽病相同的症状。对病原菌进行分子生物学鉴定,基于ITS基因片段构建的系统发育树结果显示,病原菌HNYF1与Colletotrichum gloeosporioides (ID:KT007120.1)聚为一个分支,支持率为98%;分别基于MnSOD、CHS、Cal等基因片段构建的系统发育树结果显示,病原菌HNYF1与暹罗刺盘孢(Colletorichum siamense)聚为一个分支,支持率均为100%;基于ACT基因片段构建的系统发育树结果显示,病原菌HNYF1与Colletotrichum siamense (ID:MG324082.1)聚为一个分支,支持率为96%。因此,通过形态学观察和多基因分子系统学方法,将菠萝蜜炭疽病的病原菌鉴定为胶孢炭疽菌(Colletotrichum gloeosporioides)类群中的暹罗刺盘孢(Colletorichum siamense)。

In order to identify the pathogen causing jackfruit anthracnose, in this study, the jackfruit anthracnose was isolated and purified by conventional methods, and the pathogenicity was verified by Koch’s postulate. The results showed that isolated pathogen could cause the same symptoms as field anthracnose on healthy leaves of jackfruit. Molecular biology identification of the pathogen, based on ITS gene to construct phylogenetic tree showed that the pathogen HNYF1 and Colletotrichum gloeosporioides(ID: KT007120.1) clustered into one branch,and the support rate was 98%. The phylogenetic tree constructed based on the ACT gene fragment showed that the pathogen HNYF1 and Colletotrichum siamense(ID: MG324082.1) were grouped into one branch, with an approval rating of 96%. The phylogenetic trees based on the MnSOD Cal, CHS, gene fragment were all clustered into one branch with Colletorichum siamense, and the support rate was 100%. To sum up, through the morphological features and polygenic systematics of pathogen identification, jackfruit anthracnose pathogen was Colletorichum siamense.