芒果MinMYBPA基因的克隆及载体的构建

Cloning and Constructing Vector of MinMYBPA Gene from Mango

MYB (Myeloblastosis)是植物最大的转录因子家族之一,广泛参与植物代谢调控。为了研究其在芒果果皮中花青苷合成的作用机理,本研究从芒果果皮中克隆得到一个与花青苷合成相关的基因,将其命名为MinMYBPA,其cDNA全长序列为1 081 bp,开放阅读框为843 bp,编码280个氨基酸,蛋白质分子量为32.09 k D,等电点为9.01,对MinMYBPA基因编码的蛋白进行系统发育分析,发现其与克莱门柚具有较近的亲缘关系。本研究为深入了解其在芒果花青素合成和积累的分子机理,进一步构建了pTRV2-MinMYBPA沉默表达和pGreenⅡ62-SK-MinMYBPA过表达载体,为MinMYBPA的功能鉴定提供参考依据。

MYB(Myeloblastosis) is one of the largest transcriptional factor families in plants and is widely involved in the regulation of plant metabolism. In order to study the mechanism of anthocyanin synthesis in mango peel, a gene related to anthocyanin synthesis was cloned from mango peel. It was named MinMYBPA and the full-length c DNA sequence was 1 081 bp. Its open reading frame was 843 bp, encoding 280 amino acids. The molecular weight of MinMYBPA protein was 32.09 kD and the isoelectric point was 9.01. Phylogenetic analysis of the protein encoded by MinMYBPA gene showed that it was closely related to Clement pomelo. In order to understand the molecular mechanism of anthocyanin synthesis and accumulation of MinMYBPA in mango, the silencing expression vector of pTRV2-MinMYBPA and the overexpression vector of pGreenⅡ 62-SK-MinMYBPA were constructed, which laid a theoretical foundation for the functional identification of MinMYBPA.