‘丹霞’铁皮石斛的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of 'Danxia' Dendrobium officinale

为了解决‘丹霞’铁皮石斛的市场供需矛盾,本研究利用植物组织培养技术,采用10种不同培养基培养‘丹霞’铁皮石斛种子,比较分析其种子萌发、原球茎形成和组培苗生长发育情况。结果表明,‘丹霞’铁皮石斛种子活力为89.54%,原球茎在1/2 MS+20%马铃薯培养基上的生长发育良好,种子培养49 d后形成原球茎。其株高、分蘖数、根长和根数等指标分别可达6.68 cm、1.96株、5.16 cm和5.88条,均高于其他培养基。在栽后115 d的统计成活率可达99.45%。说明培养基1/2 MS+20%马铃薯适合于‘丹霞’铁皮石斛的组培苗培养和快速繁殖。本研究为‘丹霞’铁皮石斛的工厂化育苗提供技术支撑。

In order to solve the market supply and demand contradiction of ’Danxia’ Dendrobium officinale, plant tissue culture technology was used in this study, the seed of ’Danxia’ Dendrobium officinale was cultivated on the10 kinds of culture medium to discuss the seed germination, the formation of protocorms, and the growth of tissue culture seedlings in Northern Guangdong. The results showed that the seed vigor of ’Danxia’ D. officinale in northern Guangdong is 89.54%, and the protocorm grew well on 1/2 MS+20% potato medium, and the protocorm was formed after 49 days of seed culture. The protocorms in 1/2 MS+20% potato culture medium grew best. The indexes such as plant height, number of tillers, root length and number of roots were 6.68 cm, 1.96, 5.16 cm and5.88, respectively, which were higher than other media. And the rate of survival 99.45% is the highest 115 days after transplantation. So 1/2 MS +20% potato is fit for the tissue cultured seedling of ’Danxia’ D. officinale in northern Guangdong. This study provided technical support for the industrialized seedling of ’Danxia’ D. officinale.