牡丹(Paeonia suffruticosa)胚胎培养研究进展

Research Advance of Paeonia suffruticosa Embryo Culture

种胚是牡丹组织培养的重要外植体,胚胎培养技术是牡丹遗传转化及生物技术育种的重要手段之一。本研究从基因型、胚龄、解除休眠、培养基成分等方面分析了不同因子对牡丹胚胎培养的影响,总结了牡丹胚胎培养的研究进展。并根据存在的问题,对牡丹胚胎培养未来研究的重点方法、领域及发展的方向进行了探讨,旨在建立以牡丹胚胎为外植体的高效、稳定的再生体系,为牡丹转基因研究提供参考。

Seed embryo is a vital explant of peony tissue culture,embryo culture is one of the essential means of genetic transformation and biotechnology breeding of peony.This research analyzed the effects of different factors on embryo culture in peony seedlings from the aspects of genotype,embryonic age,dormancy release,and medium component,and summarized the advances of the research in embryo culture of peony.Moreover,according to the existing problems,the key methods,fields and development directions of embryo culture of peony in the future were discussed,aiming at establishing an efficient and stable regeneration system with peony embryo as explant,as well as providing references for the transgenic study of peony.