一种新的豆瓣菜(Nasturtium officinale)锌铁转运蛋白基因的克隆及序列分析

Cloning and Sequence Analysis of a Novel Zinc Iron Transporter Gene from Nasturtium officinale

为了深入研究豆瓣菜(Nasturtium officinale R. Br.)对锌和铁的吸收和转运,我们采用RACE方法克隆了一种豆瓣菜根部锌铁转运蛋白NoZIP2 (N. officinale zinc iron transporter protein 2)的全长cDNA,并采用生物信息学方法对序列进行分析。结果表明,NoZIP2包含1 446 bp,编码一个357个氨基酸残基的多肽,包含了9个跨膜结构域,存在植物锌铁转运蛋白的保守结构域,可能参与了豆瓣菜锌铁转运的过程。NoZIP2氨基酸序列与其它物种ZIP的氨基酸序列相似性较高,其中与荠菜的相似性最高,氨基酸相似性高达92%。分子进化分析表明,NoZIP2与十字花科植物荠菜、荠蓝和小花蓝芥的ZIP能够聚到一起。从豆瓣菜根部克隆的NoZIP2具有其它植物锌铁转运蛋白相似的高度保守的ZIP家族成员序列特征,因此推测NoZIP2属于ZIP家族成员。豆瓣菜No ZIP2的克隆和序列分析能够为进一步研究其功能及表达提供理论基础。

In order to study the zinc and iron obsorption and transportation of Nasturtium officinale,we cloned a full length cDNA of zinc iron transporter from roots of N.officinale by RACE method and analyzed its sequence by bioinformatical method.The results showed that,the full length cDNA of NoZIP2 included 1 446 bp,encoded a peptide of 357 amino acids,contained 9 transmembrane domains and highly conserved zinc iron transporter signature sequences in plant,which might be involved in the process of zinc and iron transport in plants.The amino acid sequence of NoZIP2 was similar with those of other plants.Among them,C.rubella had the highest similarity,which was 92%.The phylogenetic analysis showed that NoZIP2 was clustered with those of C.rubella,C.sativa and A.alpina.NoZIP2 cloned from N.officinale had highly conserved ZIPs signature sequences from other plants,which indicated that it belongs to ZIP family.The cloning and sequence analysis of zinc iron transporter protein 2(NoZIP2)from Nasturtium officinale can provide a theoretical basis for further study on its function and expression.