龙眼Hsf基因家族密码子使用模式分析

Codon Usage Pattern Analysis of Dimocarpus longan Lour. Hsf Gene Family

为了解龙眼热激转录因子(heat shock transcription factor, Hsf)基因家族密码子使用偏性特征,本研究利用CodonW程序和EMBOSS在线软件对龙眼Hsf (DlHsf)基因家族密码子偏好性进行数据的提取及分析,运用SPSS、Origin和GraphPad软件对密码子偏好性参数进行统计、分析与图表制作。结果显示:(1) DlHsf基因家族密码子A/T (U)选择偏性较G/C更强,且更偏好以A/T (U)结尾。(2)最优密码子有CUU等12个。(3)密码子组成成分分析、中性绘图分析、ENC-plot分析及PR2-plot分析表明,DlHsf基因家族密码子偏好性主要受到突变压力影响。(4)与模式生物基因组密码子使用偏性使用频率比较显示,酵母菌真核表达系统更适合作为该家族基因异源表达系统,拟南芥、烟草、番茄均可作为该家族遗传转化的受体,但为提高表达效率,可能仍需在实验过程中对家族基因密码子进行改造优化。本研究为DlHsf基因家族密码子优化及转基因功能尤其是在异源表达上的研究提供一定的理论基础。

In order to understand the bias characteristics of codon usage of heat transcription factor(Hsf)gene family in%Dimocarpus longan Lour.this study used CodonW program and EMBOSS online software to extract and analyze the codon preference of Dimocarpus longan Lour.Hsf(DlHsf)gene family.Statistics,analysis and charting of codon preference parameters have been used by the software of SPSS,Origin and GraphPad.The results showed four points:(1)The DlHsf gene family codons A/T(U)selection bias were stronger than G/C,and it was more preferred to end with A or T(U);(2)There were 12 optimal codons of CUU;(3)Codon composition analysis,neutral mapping analysis,ENc-plot analysis,and PR2-plot analysis indicated that the DlHsf gene family codons preference was mainly due to mutational stress;(4)Compared with the pattern bio-genome codon usage bias,the yeast eukaryotic expression system was more suitable for heterologous expression system of this family.Arabidopsis,Nicotiana sylvestris and Solanum lycopersicum always can be used as a better receptor for genetic transformation of this family members,but during the experiment,the modification and optimization of codons is necessary.The results of this study provide a theoretical basis for the study of transgenic functions and codon optimization of DlHsf gene family.