桑树bHLH转录因子家族全基因组鉴定与分析

Genome-wide Identification and Analysis of the bHLH Transcription Factor Gene Family in Morus notabilis

本研究旨在鉴定桑树bHLH基因,并分析预测其理化性质和保守结构域,以期为研究bHLH转录因子在桑树的生长发育、抗逆胁迫以及信号转导过程中的作用提供参考。本研究使用桑树全基因组数据,通过生物信息学方法对桑树的b HLH转录因子进行了鉴定与系统分析。结果表明,在桑树全基因组中共鉴定出173个b HLH转录因子,经过系统进化分析,将其分为四个进化大组,11个亚组;bHLH转录因子间理化性质差别和跨度较大,59.1%的等电点小于7,呈弱酸性;保守结构域分析表明,bHLH转录因子有两个高度保守的结构域并含有H5-E9-R13保守序列,N端的碱性氨基酸区与DNA结合位点有关,C端的HLH区形成螺旋-环-螺旋结构并且与形成二聚体有关,共含有5种保守元件;保守元件分析表明,bHLH基因家族只含有一个HLH保守结构域结构;蛋白互作分析结果表明,相似的功能或者相近的生理过程可能由不同的蛋白控制。鉴定和分析桑树bHLH转录因子家族,既可以为桑树的抗旱、胁迫等方面的研究提供分子生物学理论基础,又可以为分子改良桑树品种提供理论依据,从而改良桑椹的果实品质,进一步提高桑椹的食用价值和药用价值。

The aim of this study w as to identify the bHLH gene of Morus notabilis,and to analyze its physicochemical properties and conserved domains,in order to provide a reference for studying the role of bHLH transcription factors in Morus notabilis growth and developm ent,stress resistance and signal transduction.Therefore,this study used Morus notabilis genome-wide data to identify and systematically analyze the bHLH transcription factor of Morus notabilis by bioinformatics.The results showed that 173 bHLH transcription factors were identified from the whole genome of Morus notabilis.After phylogenetic analysis,they were divided into four evolutionary groups and 11 subgroups;the difference between physicochemical properties and span of bHLH transcription factors is large,and the 59.1%theoretical pI is less than 7,which is weakly acidic;conserved domain analysis indicates that bHLH transcription factor has two conserved domains and contains H5-E9-R13 conserved sequence,the N-terminal basic amino acid region is associated with the DNA binding site.The C-terminal HLH region forms a helix-loop-helix structure and is associated with the formation of dimers,which contain five conserved elements;conservative element analysis showed that the bHLH gene family contains only one HLH conserved domain structure;The results of protein interaction analysis showed that similar functions or similar physiological processes may be controlled by different proteins.Identification and analysis of the Morus notabilis bHLH transcription factor family can provide molecular basis for the research of drought resistance and stress of Morus notabilis,and lay a foundation for molecularly modified Morus notabilis varieties,thereby improving the fruit quality and further improving the edible value and medicinal value.