沙棘转录因子基因HrWRKY1的克隆与表达分析

Cloning and Expression Analysis of Transcription Factor Gene HrWRKY1 in Seabuckthorn(Hippophae rhamnoides)

沙棘是中国干旱、半干旱地区重要生态经济林木,WRKY基因家族是调控植物抵御生物与非生物胁迫重要转录因子。本研究利用沙棘转录组数据,克隆并分析转录因子基因HrWRKY1。结果发现,HrWRKY1开放阅读框1 320 bp,编码439个氨基酸残基,蛋白分子量49.26 kD,等电点5.55,属于WRKY转录因子Group I;进一步分析该基因在不同逆境胁迫下的表达,结果发现,HrWRKY1参与氯化钠(sodium chloride, NaCl)、聚乙二醇(polyethylene glycol, PEG)、脱落酸(abscisic acid, ABA)与低温等非生物胁迫的响应,说明其与沙棘多种非生物胁迫密切相关。本研究结果将为今后研究沙棘WRKY转录因子家族基因功能提供参考,并为林木抗逆研究及分子育种提供科学依据。

Seabuckthorn is an important ecological and economic forest in arid and semi-arid regions of China.The WRKY gene family is an important transcription factor that regulates plants against biotic and abiotic stresses.This study used the seabuckthorn transcriptome data to clone and analyze the transcription factor gene HrWRKY1.The results showed that the open reading frame of HrWRKY1 was 1 320 bp,encoding 439 amino acid residues,with a molecular weight of 49.26 kD and an isoelectric point of 5.55.It belongs to WRKY transcription factor Group I;further analysis of the expression of this gene under different stresses,it was found that HrWRKY1 is involved in the response of abiotic stresses such as sodium chloride(NaCl),polyethylene glycol(PEG),abscisic acid(ABA)and low temperature,indicating that it is closely related to various abiotic stresses of seabuckthorn.The results of this study will provide a reference for the future study of WRKY transcription factor family gene function of seabuckthorn,and provide scientific basis for forest stress resistance research and molecular breeding.