牡丹种胚离体再生体系建立

Establishing Regeneration in vitro System of Tree Peony Embryos

牡丹(Paeonia suffruticosa Andr.)为多年生落叶灌木,其种子油的不饱和脂肪酸含量达90%以上,尤其是α-亚麻酸,含量超过40%,是一种非常适合人类食用的油脂。本研究以牡丹种胚为外植体进行愈伤组织诱导和胚萌发,确定胚的最适成熟度,并筛选最适的愈伤分化及丛生芽诱导和再生植株生根培养基,建立牡丹种胚离体再生体系。结果表明:幼胚的诱导率比成熟胚高,污染率和褐化率比成熟胚低,幼胚更适用于牡丹离体再生;愈伤分化和丛生芽诱导的最适培养基为添加6-BA 1.0 mg/L,KT 1.0 mg/L的MS培养基,芽诱导率为37.67%,丛生芽诱导率为56.53%;促进生根的最适培养基为添加IBA 1.0 mg/L,NAA 0.5 mg/L的1/2MS培养基,生根率为26%。本研究初步建立牡丹种胚离体再生体系,为牡丹的遗传转化、基因功能研究和规模化种植提供了技术支撑。

Tree Peony(Paeonia suffruticosa Andr.)is a perennial deciduous shrub.Its seed oil contains more than90%of unsaturated fatty acids,especiallyα-linolenic acid,which is more than 40%.Peony seed oil is very suitable for human.In this study,callus induction and embryo germination were carried out with peony embryos as explants.The optimal maturity of embryos was selected,the optimal callus differentiation and multiple shoots induction and regeneration rooting medium were selected to establish peony embryos regeneration system in vitro.The results showed that the induction rate of young embryos was higher than that of mature embryos,and the pollution rate and browning rate were lower than that of mature embryos.The young embryos were more suitable for in vitro regeneration of peony.The optimum medium for callus differentiation and shoot induction was 6-BA1.0 mg/L,KT 1.0 mg/L in MS medium.The bud induction rate was 37.67%and the shoot induction rate was56.53%.The optimum medium for rooting promotion was IBA 1 mg/L,NAA 0.5 mg/L in 1/2 MS medium,and the rooting rate was 26%.This study initially established an in vitro regeneration system of peony embryos,which provided technical support for the genetic transformation,gene function analysis and large-scale planting of peony.