基于SSR标记的60份香料烟种质资源分析

Analysis of Germplasm Resources of 60 Oriental Tobacco based on SSR Marker

为充分发掘和利用香料烟种质资源、合理选配亲本、建立香料烟分子标记辅助育种体系,本研究利用分布于烟草24个连锁群上的35个SSR标记分析了60份源自11个不同国家香料烟种质资源的遗传多样性。结果显示35对SSR引物在60份种质资源中共检测出447个基因位点,观测杂合度(Ho)均值为0.623 1,预期杂合度(He)的均值为0.708 2,Shannon’s信息指数(均值, I=1.415 9)和Nei’s多样性指数(均值, H=0.694 0)较高,且遗传距离变化范围较大(0.009~0.924)。说明60份香料烟种质资源的遗传差异较大、遗传多样性丰富、亲缘关系较远。聚类分析表明,以遗传距离0.485为界可将全部种质资源划分为3个类群,且种质资源间聚类与其地理来源有很高的相关性。

In order to explore,identify,innovate and utilize of oriental tobacco germplasm resources,the genetic diversity of 60 oriental tobacco germplasms from 11 different countries was first analyzed by 35 SSR markers which evenly distributed on 24 linkage groups of tobacco.The results showed that 447 genetic loci existed in 60 oriental tobacco germplasms using 35 SSR primer pairs.The average observed heterozygosity(Ho)was 0.6231,and the average expected heterozygosity(He)was 0.708 2.The average Shannon’s information index(I=1.415 9),the average Nei’s diversity index(H=0.694 0)and the range of genetic distance(0.009~0.924)were relatively high,which indicated that the genetic variation of 60 materials was quite large and the genetic diversity was much abundant in oriental tobacco.The result of cluste r analysis revealed that 60 materials could be clustered into 3 groups at the level of GD=0.485,and which was highly correlated with their geographical origins.