荧光定量PCR检测HBVDNA与血清HBV标志物的关系

The Relationship between the Quantitative Detection of HBVDANby FQ-PCR and Serum Markers of Hepatitis B Virus

目的 探讨荧光定量 PCR检测血清 HBVDNA与血清 HBV标志物的关系。方法 采用酶联免疫吸附法(ELISA)和荧光定量 PCR(FQ- PCR)两种方法同时检测 357份肝炎患者血清 ,并对结果进行对比分析。结果 肝硬化组HBVDNA含量明显低于 HBs Ag携带者组 (P<0 .0 5)。血清 HBs Ag、抗 - HBc、HBe Ag阳性组 HBVDNA含量明显高于HBs Ag、抗 - HBc、抗 - HBe和 HBs Ag、抗 - HBc阳性组 (P<0 .0 1 ) ,而后两组 HBVDNA检出率仍较高。结论 FQ- PCR可真实反映 HBV感染、复制及病程变化 。

Objective To investigate the relationship between the quantitative detection of HBVDNA by fluorescence quantitative PCR(FQ PCR) and serum markers of hepatitis B virus.Methods 357 serum samples of patients with viral hepatitis were tested by FQ PCR assay and ELISA.Results The concentrations of HBZVDNA in cirrhosis group were much lower than those of HBsAg carriers group(P<0.05).The concentrations of HBVDNA in HBsAg,HBeAg,anti HBc positive groups were obviously higher than those in HBsAg,anti HBc,anti HBe and HBsAg,anti HBc positive groups(P<0.01),while the positive rate of HBVDNA was still high in the latter two groups.Conclusion FQ PCR could be used to actually monitor the state of HBV's infection,replication and the course of disease.