摘要
目的 :建立反相高效液相色谱法测定苓桂术甘方 (茯苓 ,桂枝 ,白术 ,甘草 ,等 )中肉桂酸的含量。方法 :HypersilC18柱 ,流动相甲醇 乙腈 水 醋酸 ( 10∶2 2∶70∶1.4 ,v/v) ,流速 0 .8mL·min-1,检测波长 2 5 4nm ,选用对二甲胺基苯甲醛作为内标物。结果 :平均加样回收率为 98.2 % ,RSD =1.9% (n =6 ) ,线性范围 8.4～ 16 8μg·mL-1,r =0 .9999。结论 :该方法简便、快速、准确、重现性好 。
Objective: To establish a method for the determination of cinnamic acid in Lingguizhugan Decoction (Poria, Ramuls Cinnamomi, Rhizoma Atractylodis macrocephalae, Radix Glycrrhizae, etc.). Methods: Using RP HPLC with Hypersil C 18 column, methanol acetonitrile water acetic acid (10∶22∶70∶1.4,v/v) as mobile phase, detection wavelength at 254nm and p dimethylaminobenzaldehyde as the internal standard.Results: The mean recovery and RSD were 98.2% and 1.9% ( n =6), respectively, the linear range of cinnamic acid was 8.4～168μg·mL -1 ( r =0.9999). Conclusion: The method is simple, rapid, accurate and reproducible, and may be used for the determination of cinnamic acid in Lingguizhugan Decoction.
出处
《中成药》
CAS
CSCD
北大核心
2002年第10期764-765,共2页
Chinese Traditional Patent Medicine
