摘要
目的 :观察苦参碱对培养SD大鼠心肌细胞内钙浓度的影响 ,以进一步明确苦参碱抗心律失常的机理。方法 :利用培养的乳鼠心肌细胞 ,负载Fura 2 /AM ,在不同的条件下 (培养液中分别加维拉帕米、氯化钾、不同剂量的苦参碱等 ) ,应用图象分析系统 ,测定心肌细胞内钙荧光强度的变化 ,间接测定苦参碱对钙内流的影响。结果 :高钾环境下心肌细胞内钙荧光强度增高 ,而苦参碱对此有拮抗作用。结论
Objectives: To clarify the antiarrhythmia mechanism of matrine by observing its effects on intracellular calcium concentration in cultured myocardial cells of SD rats. Methods: Myocardial cells of newborn(sucking) rats were fed with culture medium supplemented with verapamil、 potassium chloride and different dose of matrine, respectively. By means of the image analysis system, intensity changes of intracellular calcium were determined with the fluorescent probe to estimate indirectly inward calcium influx under the influence of matrine. Results: It was found that high potassium caused an increase in fluorescent intensity of intracellular calcium which could be inhibited by matrine. Conclusion: It is suggested that matrine may be a kind of calcium antagonium producing its antiarrhythmic action.
出处
《中成药》
CAS
CSCD
北大核心
2002年第10期774-776,共3页
Chinese Traditional Patent Medicine
基金
上海市高等学校科学技术发展基金资助项目 (No .98C12 )
