IFN-γ抗结核分枝杆菌感染的实验观察

Experimental Observation of INF-γ on Resistance to Mycobacterium Turbercuiosis Infection

目的 观察IFN -γ抗结核分枝杆菌感染的作用。方法 将BALB/c小鼠制成结核分枝杆菌感染模型 ,随机分为 4组 ,分别以IFN -γ、LVFX、IFN -γ +LVFX及PBS治疗 ,检测重要脏器菌落数 ,观察小鼠平均生存时间。结果 各治疗组肝、脾、肺组织菌落数均显著低于对照组 ,尤其IFN -γ +LVFX治疗组效果更为明显 ;治疗组小鼠平均生存期均显著高于对照组 (P <0 .0 1)。结论 外源性IFN -γ可减少肝、脾、肺组织中结核分枝杆菌菌落数 ,延长动物生存时间 ,促进Th1细胞的免疫应答 ,在抗结核分枝杆菌感染中起着重要作用。

Objective To study the effect of IFN-γ on resistance of BALB/c mice to Mycobacterium tuberculosis infection.Methods The BALB/c mice infected with Mycobacterium tuberculosis were randomly divided into four groups and were treated with IFN-γ,LVFX,IFN-γ+LVFX and PBS,respectively.The colonies of bacteria in major organ were counted and the survival times of the mice were observed.Results In three treated groups the bacterial colonies in liver,spleen and lung were markedly reduced than that in control group,especially in IFN-γ+LVFX combined treated group.In the treated groups the mean survival time of the mice was longer than that in the control group ( P <0.01).Conclusion This study shows that exogenous IFN-γ could decrease the bacterial colonies in liver,spleen and lung,extend the survival time of mice infected with Mycobacterium tuberculosis, and promote immune response of Thl cells.It suggests that IFN-γ could play an important role in anti-tuberculosis protection in mice.