水稻黄矮病毒的纯化和病毒蛋白质及RNA组分的分析

PARTIAL PURIFICATION OF RICE YELLOW STUNT VIRUS AND CHARACTERIZATION OF VIRUS STRUCTURAL PROTEINS AND RNA GENOME

从人工接种水稻黄矮病毒(Rice yellow stunt virus,RYSV)的水稻茎杆中提取到部分纯化的RYSV制品。分析了RYSV的蛋白质组分,表明它的结构蛋白含有L(170k)、G(84k)、N(60k)、M1(31k)、M2(29.5k)和一个分子量为42k的蛋白,并测定了G蛋白氨基末端的部分氨基酸序列。经测定,RYSV RNA的分子量约为12kb。

Partially purified RYSV preparation was obtained by extraction from stalks of artificially infected rice plants. Analysis of RYSV structural proteins by SDS-PAGE revealed that it consisted of 5 protein species, L(l70k), G(84k), N(60k), Ml(3lk)and M2 ( 29.5k ),in addition to a protein with molecular weight of 42k. Furthermore, the N terminal amino acid sequence of 15 residues for G protein was determined by automated Edman degradation method.The size of RYSV RNA genome was estimated to be about 12 kb.