炭疽芽孢杆菌噬菌体AP631的生物学特性检测

CHARACTERISTICS OF BACILLUS ANTHRACIS BACTERIOPHAGE AP631

炭疽病(Anthrax)一直是分布较广、发病率较高的烈性传染病。病原菌的分离和鉴定是诊断炭疽病的关键手段。用特异噬菌体进行炭疽杆菌(Bacillus anthracis)的鉴别具有快速、准确、简便等优点。董树林等于1963年分离到炭疽芽孢杆菌噬菌体AP631,从60年代就开始用于实验诊断,对其生物学特性曾进行部分研究。但目前对AP631所知甚少。本实验的目的在于为病原学诊断和AP631的进一步利用,提供数据和积累资料。

The biological characteristics of phage AP631, the Anthrax diagnostic phage used in China, was studied. It has a hexagonal head of around 50 nm and a long noncontractile tail of about 180nm with a double stranded DNA of about 49kb. AP631 could not 1yse encapsulated B. anthracis, and also, no phage was adsorbed on the surface of encapsulated strain under the electron microscope. For diagnostic purpose, it is important to cultivate the bacteria under non-capsulation conditions.