摘要
用经C6/36细胞传代的登革热病毒制备抗原,建立了捕捉法ELISA检测特异性IgM抗体用于快速诊断登革热病人。临床和流行病学符合登革热诊断的72份病人血清,在发病后1～3日内用本法检测,IgM阳性检出率为35％(7/20),4～7日内为69％(18/26),8～16日内为95.8(23/24)。另2份病程30天的病人血清,仍可检出IgM抗体。本方法敏感性高,重复性好,特异性强,未发现与乙型脑炎抗体有交叉反应,而且具有省时、快速等优点,是简便、可靠的诊断登革热的方法。
A Mac-ELISA for rapid diagnosis of dengue infection was developed. The dengue virus antigen was prepared in C6/36 cell line. 72 cases sus cepted of dengue fever in the clinic were tested, by days after onset of illness. The positive rates were as follows. 1-3th day 35%; 4-7th day 69%; 8-16th day 95.3%. In two other cases the IgM antibody was still positive 30 days after the onset of illness. There was no cross reaction between Japanese B encephalitis and dengue fever in IgM tests.
出处
《病毒学报》
CAS
CSCD
北大核心
1992年第4期349-353,共5页
Chinese Journal of Virology
关键词
登革热
ELISA
诊断
IgM antibody captur ELISA Dengue fever Rapid diagnosis
