地黄不同品种光和色素含量及其与叶色的关系

Photosynthetic Pigment Contents of Different Germplasm of Rehmannia glutinosa and the Relationship between Pigments and Leaf Color

目的 :应用光和色素含量评价地黄的种质资源。方法 :选 2 0个地黄品种 ,在相同条件下栽培 ,8月 2 3日和 9月 2 5日每个品种分别于不同植株上取 3张叶片 ,于上部侧脉间取 80mg叶肉 ,于 96 %乙醇中研碎提取 ,上清液用于测定吸光度。结果 :不同品种叶片光和色素含量差异达极显著水平 (P≤ 0 .0 1)。两次测定结果相似。叶绿素a含量最高 ,且品种间含量变化较大 ,应用邓肯法进行多重比较 ,可根据叶绿素a含量将 2 0个品种分为 9个差异显著的区组。此外 ,叶绿素a与叶色之间具显著的相关关系 ,其含量高者叶色为深绿色 ,低者为黄绿色或灰绿色。结论 :光和色素含量高低是地黄栽培品种的特征之一 ,是地黄种质资源评价的重要指标。对于地黄品种的鉴定具有一定的应用价值。

Objective: To evaluate the germplasm of Rehmannia glutinosa on the basis of photosynthetic pigment contents (PPC). Method: 20 cultivars were planted on the same condition. On Oct. 23 and Sept. 25, 3 leaves per cultivar were collected on different plants, and 80 mg mesophyll was collected among upper lateral veins and was ground in 96% alcohol, and the supernatant was subjected to measure on a spectrophotometer (Angilent 8453). Result: The PPCs among cultivars were significantly different at a P≤0.01 level. The results of the measurements were similar. Chlolophyll a was the most abundant pigment, but varied to a great extent among different cultivars. 20 cultivars were divided into 9 homogeneous groups according to the contents of chlorophyll a by Duncan's multiple range test at P≤0.05. In addition, the content of chlorophyll a was closely related to leaf color. The cultivars with higher chlolophyll a had deep green leaves, and those with lower had yellow green or pale green leaves. Conclusion: PPC was an inherent character and an important index for the germplasm evaluation of R. glutinosa.