葛根提取物对正常及去势大鼠血清雌激素水平影响的研究

Effect of the Purariae-isofiavones on Estrogen Level in Normal and Ovariectomized Rats

目的 :研究葛根提取物对大鼠雌激素水平的影响。方法 :将 80只成年雌性大鼠随机分成 4组 ,每组2 0只 :正常对照组 ;正常 +灌胃葛根提取物 ,30d ;去势组 ,去势 +灌胃葛根提取物 ,30d。抽血测定血清E0 ,E2 ,E3 ,睾酮等性激素水平 ;FSH ,LH ,PRL ,GH等促性激素水平。结果 :去势后 ,E2 ,E3 显著下降 (P <0 .0 5 ) ;E0 有所下降 ,但差异无显著性 (P >0 .0 5 ) ,睾酮以及FSH ,LH ,PRL ,GH等垂体分泌激素增高 (P <0 .0 5 )。葛根提取物灌注后 ,正常组雌激素和促性激素水平均有不同程度的下降 ;去势动物的E2 ,E3 恢复正常 ;E0 和睾酮比正常组增高 (P <0 .0 5 ) ,但睾酮水平与去势组比较差异无显著性 (P >0 .0 5 )。结论 :葛根提取物可恢复去势大鼠的雌激素水平 ,提高促性激素水平 ;在正常大鼠体内 ,该物质表现为抗雌激素作用。

Objective: To study the effect of purariae isoflavone on estrogen level in ovariectomized rats.Method:80 rats were divided into four groups randomly, every group with 20 rats:1.Control group;2.Normal + purariae isoflavone group; 3.Ovariectomized group; 4.Ovariectomized + purariae isoflavone group. Estrogen level and gonadotropin-releasing hormon level of all rats were measured. Result:Thirty days after being ovariectomized , E 2, E 3 level was signifcantly lower than that of the first group(P<0.05) .But Testerone , FSH,LH,PRL and GH increased(P<0.05).After being gastrogavaged with purariae-isoflavone for thirty days, Estrogen level and gonadotropin-releasing hormon level of the second group were significantly lower in various degree than those of normal control group (P<0.05). But in ovariectomized rats, the estrogen level was recovered (P>0.05). The gonadotropin-releasing hormon level was increased (P<0.05). Conclusion: Purariae-isoflavone can increase estrogen level to normal in ovariectomized rats by way of increasing the level of gonadotropin-releasing hormon. In normal rats, it has anti-estrogen effect.