知母合黄芪改善肾性高血压大鼠心功能的研究

Study on the Efect of Zhimu Combined Huangqi on Improving Renal Hypertension Rat's Cardiac Dysfunction

目的 :观察知母、黄芪单用及合用对实验性心功能障碍的改善作用 ,重点观察知母抑交感、β阻滞作用和黄芪改善血流动力学作用对心衰的影响。方法 :大鼠行两肾一夹术后造模 8周 ,分组给药 6周后 ,测定心率和血压 ,左心室插管测定心功能后取心脏测心重指数和室重指数 ,测定血浆儿茶酚胺和cAMP含量以及心肌组织cAMP含量。结果 :知母可降低心率 ,改善心室重构 ,但对心功能影响不明显 ;黄芪能降低血压 ,明显升高 +dp/dtmax及 dp/dtmax水平 ;两药合用在儿茶酚胺和cAMP含量及心功能多个指标上改善作用显著 ,效果更好。结论 :知母和黄芪对实验性心功能障碍模型有保护作用 ,且合用优于单用 ,说明两药协同治疗心功能障碍的效果较好。

Objective:To observe the effect of Zhimu and Huangqi used singly or combinatively on improving experimental cardiac dysfunction, and mainly to observe zhimu's effect on restraining sympathetic nerve and blocking β-adrenergic-recepter and huangqi's effect of improving hemodynamics on heart failure. Method:Two-clib one kidney operation was done to make renal hypertension rat model, 8 weeks after operation, rats were divided into groups and medicatied for 6 weeks, and then their heart rate and blood pressure were measured, left ventricle was cannulated to estimate heart function, and heart-weight-index and left-ventricle-weight-index were measured. Result:Zhimu could slow rats heart rate obviously, prevent cardiac remodeling, but did not affect cardiac function remarkably; Huangqi could reduce blood pressure, heighten +dp/dt max and -dp/dt max remarkably; the combined use of the two drugs could decrease plasma catecholamine concentration, adjust myocardium cAMP content ,and improve heart fuction obviously. Conclusion:Zhimu and Huangqi can protect experimental cardiac dysfunction, and the combined use is better than the single use, which shows it betler to use the two drugs combinatwely in treating heart dysfunction.