丹参葡萄糖注射液的HPLC指纹图谱研究

Studies on HPLC Fingerprint of Danshen Glucose Injection

目的研究丹参葡萄糖注射液的HPLC指纹图谱测定方法。方法以原儿茶醛为对照品,应用HPLC法,色谱柱:AlltimaC18(4.6mm×250mm,5μm);流动相:1%冰醋酸水∶1%冰醋酸甲醇,梯度洗脱;检测波长:281nm;分析时间:65min;流速:1.0mL/min;结果标示出丹参葡萄糖注射液6个共有峰,并用HPLC/MS对主要成分进行了归属。结论该方法准确、重复性好,为丹参葡萄糖注射液的指纹图谱质量控制提供了依据。

Objective: To establish the fingerprint of Danshen Glucose Injection by HPLC. Methods: Separation was performed on an Alltima C18(4.6mm×250mm,5μm) analytical column, with mobile phase consisting of 1 %acetic-water and 1 %acetic acid-methanol and with gradient elute at the flow rate of 1mL/min-1. The UV detection was set at 281nm. Results: Six peaks of Danshen Glucose Injection wre indicated, and the chief ingredients were determined by HPLC. Conclusion: This method is accurate and with good reproducibility and can be used for the quality control of Danshen Glucose Injection.