冷冻对G422胶质瘤HSP90α和P53基因表达的影响

The effects of Cryodestruction on the expression of HSP90αand P53 gene in glioma

目的 :研究冷冻后G4 2 2胶质瘤HSP90α和P5 3基因表达的变化及两者之间的关系 ,探讨其变化在冻化疗联合应用中的意义。方法 :G4 2 2胶质瘤接种于小鼠后下肢肌腹内 ,长到 2cm大小随机分组实施冷冻 (- 180℃冷冻 90s) ;免疫组化方法检测HSP90α和P5 3的冻前及冻后 6、12、2 4、4 8、72h的表达。结果 :HSP90α的表达在冻后 6h显著低于对照组 (P <0 .0 1) ,冻后 2 4、4 8、72h其表达显著增高 ;P5 3基因表达于冻后各时点均增高 ,尤以冻后 2 4h和 4 8h最为显著 (P <0 .0 1)。结论 :冷冻影响G4 2 2胶质瘤的HSP90α及P5 3的表达 ,两者的表达呈显著正相关 ;推测冻后两者的表达变化在冻化疗联合应用中起到关键性调节作用。

Objective To study the expression of the HSP90α and P53 gene in glioma after cryodestruction,their relation; and to disscuss the effects on cryochemotherapy about their changes.Methods G422 glioma (6.5～7.5 10 5 /mouse) was inoculated into the muscle of the hind limbs of the mice;Approximately 2 cm in diameter (9 days, after the inoculation),cryodestruction (freezing for qo sec at -180 ℃) was performed; The expression of the P53 and HSP90α gene were investigated by immunohistochemistry in 6?12?24?48 and 72 hours after cryodestruction respectively.Results The expression of the HSP90α was significantly lower than that of the control group in 6 hours but higher than in 24?48 and 72 hours after cryodestruction( P <0.01);The expession of the P53 gene was higher than that of the control group at any time points, especially in 24 and 48 houes after cryodestruction ( P <0.01).Conclusion Cryodestruction has an important effect on the expression of the HSP90α and P53 gene. It is suggested that their expressions in glioma after cryodestruction have a key regulatary function in cryochemotherapy.