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抗组织蛋白酶B单克隆抗体可变区基因的克隆和序列分析 被引量：1

DNA SEQUENCE ANALYSIS OF VARIABLE REGION GENES FROM 2A2 MONOCLO-NAL ANTIBODY AGAINST CATHEPSIN B
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摘要 目的 :为制备基因工程抗体 ,需要对组织蛋白酶 B单抗可变区基因的核苷酸序列进行分析。方法 :利用 RT- PCR技术扩增 2 A2抗组织蛋白酶 B单克隆抗体可变区基因 ,测序并和已报道的小鼠免疫球蛋白的基因库进行比较。结果 :2 A2抗组织蛋白酶 B单克隆抗体可变区基因的克隆和序列分析及比较结果没有发现有害的基因突变。结论 :2 Objective:In order to prepare an enineering antibody derived from 2A2 murine monoclonal antibody against cathepsin B, we need to analyze the DNA sequence of variable region genes Method:RT PCR was used in amplification of variable region genes of 2A2 monoclonal antibody Those genes were cloned and subjected to nucleotide sequence analysis and compared to the murine immunoglobulin germline sequences for possible somatic hypermutations Result:No harmful mutations were found Conclusion:Variable region genes of 2A2 the monoclonal antibody are suitable for the construction of mouse/human chimeric antibody
作者 樊晓晖 Kopitar-Jerala Natasa Ales Premzl Janko Kos
机构地区 广西医科大学基础学院微生物学与免疫学教研室 Department of Biochemistry and Molecular Biology Jotzef Stear Institate
出处 《广西医科大学学报》 CAS 2002年第5期628-630,共3页 Journal of Guangxi Medical University
关键词 组织蛋白酶B 单克隆抗体 可变区基因 序列分析 克隆 cathepasin B monoclonal antibody variable region genes sequence
分类号 Q78 [生物学—分子生物学]
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参考文献13

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同被引文献15

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广西医科大学学报
2002年 第5期

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