大剂量维生素C在急性胰腺炎患者脂质过氧化损伤中的保护作用

The Effect of High-dose Vitamin C on Lipid Peroxidization Injury in Patient with Acute Pancreatitis

目的:观察大剂量维生素C(Vit.C)对急性胰腺炎患者血浆过氧化脂质变化的影响,探讨其对脂质过氧化损伤的保护作用。方法:将84例胰腺炎病人随机分为治疗组和对照组。治疗组应用Vit.C 10g稀释于5％葡萄糖500ml中静脉滴注,每日1次,连用5天;对照组应用Vit.C 1g稀释于5％葡萄糖500 ml中静脉滴注,每日1次,连用5天。另取40例健康成年人作为正常对照组。观察两组患者治疗前后血浆维生素C(P-VC)、过氧化脂质(P-LPO)、血浆维生素E(P-VE)、血浆-β胡萝卜素(P-β-CAR)、全血还原性谷胱甘肽(WB-GSH)、红细胞超氧化物歧化酶(E-SOD)、红细胞过氧化氢酶(E-CAT)的含量变化。结果:急性胰腺炎患者与正常人群相比较,P-VC、P-VE、P-β-CAR、WB-GSH、E-SOD、E-CAT的平均含量明显降低(P<0.05),而P-LPO显著增高(P<0.05)。且以上各指标的变化急性重症胰腺炎(SAP)组较急性轻症胰腺炎(MAP)组为明显(P<0.05)。治疗组经治疗后,P-VC、WB-GSH、E-SOD、E-CAT较对照组明显升高(P<0.05),而P-LPO明显降低(P<0.05)。结论:大剂显Vit.C能提高胰腺炎患者的抗氧化能力,阻断血浆中正在进行的脂质过氧化过程,对脂质过氧化过程有一定的保护作用。

Purpose: To observe the effect of high-dose vitamin C(Vit. C) on lipid peroxidation injury in acute pancreatitis(AP) patients. Methods: 84 cases of AP patients were divided into treated group and controlled group, and 40 healthy cases were taken as normal group. In the Vit. C treated group, Vit. C (10 g/d) was given intravenously for 5 days. In the controlled group, Vit. C(1 g/d) was given intravenously for 5 days. The concentration of plasma vitamin C(P-VC) , plasma lipid peroxidation(P-LPO), plasma vitamin E(P-VE), plasma β-carotene (P-β-CAR), whole blood glutathione(WB-GSH) and the activity of erythrocyte surperoxide dimutase(E-SOD), erythrocyte catalase(E-CAT) were measured by spectropho-tometry in the normal group and before and after treatment with Vit. C in the treated group and the controlled group. Results: Compared with the normal group, the average value of P-VC, P-VE, P-β-CAR, WB-GSH and the activity of E-SOD, E-CAT of the AP patients were obviously decreased and the average value of P-LPO was obviously increased, especially in the acute severe pancreatitis (SAP) patients (P< 0.05). After treatment with Vit. C the average value of P-VC, WB-GSH and the activity of E-SOD, E-CAT of the treated group were significantly higher than that of controlled group, and the value of P-LPO was lower than that of the controlled group(P<0.05). Conclusion: Intravenous infusion of a high-dose of Vit. C as antioxidants can clean abnormal free radicals and suppress the peroxidation reaction, and protect the AP patients from lipid peroxidation injury.