复方SZ滴眼液对实验性半乳糖白内障的防治作用

Protective effect of Co-SZ eye drop on galactose cataract in rats

目的 :探讨复方SZ滴眼液对实验性半乳糖白内障的防治作用。方法 :以民间验方为基础、以水蛭(SZ)为主要成分 ,配制成SZ滴眼液 ;并在SZ滴眼液的基础上配制成富含锌和维生素C的复方SZ滴眼液。用SD大鼠复制D -半乳糖白内障模型。将实验分成 3组 :①生理盐水对照组 ;②SZ组 ;③复方SZ组。用FS - 3V裂隙灯显微镜动态观察各组动物晶体混浊情况 ,并于实验第 15d测定各组晶体的超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH -Px)、谷胱甘肽 (GSH)和可溶性蛋白 (SP)的含量。观察并比较两种滴眼液对半乳糖白内障的防治作用。结果 :复方SZ组和SZ组大鼠晶体混浊的速度均较对照组慢、程度也较轻 ;复方SZ组大鼠晶体混浊的速度较SZ组慢、程度也较SZ组轻。晶体抗氧化指标测定显示 ,复方SZ组和SZ组晶体的SOD、GSH -Px和GSH含量均明显高于对照组 ,而复方SZ组晶体的SOD和GSH -Px又较SZ组高。各组晶体SP含量无显著差异。结论 :SZ滴眼液和复方SZ滴眼液均具有较好的延缓和减轻半乳糖白内障的作用 ,复方SZ滴眼液较SZ滴眼液的作用更明显。其机理可能与复方SZ滴眼液较SZ滴眼液更富含微量元素锌和维生素并具有更强的抗氧化能力有关。

AIM: To investigate the effects of Co-SZ eye drop on galactose cataract in rats. METHODS: Based on folk remedy, SZ eye drop was made from leech, as a modified SZ eye drop, Co-SZ eye drop was enriched in Zinc and Vitamin C. In the present study, animal model of galactose cataract in SD rats was used. All animals were randomly divided into 3 groups : control group(using 0.9 % NaCl instead of SZ and Co SZ), SZ group and Co-SZ group. Lens opacities were examined dynamically in each groups via FS-3V slit-lamp microscope. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH) and soluble protein (SP) in the lenses were measured in 15 days. RESULTS: Both the Co-SZ and SZ eye drops could significantly delay and alleviate galactose cataract in rats, with better effect of Co-SZ than SZ eye drop. The antioxidant index indicated that SOD, GSH-Px, GSH in Co-SZ and SZ group were significantly higher than that in control group. Furthermore, SOD, GSH-Px in Co-SZ group were higher than that in SZ group significantly. CONCLUSION: Co-SZ eye drops could significantly delay and alleviate galactose cataract in rats, the effect is much better than SZ eye drops. The different effect between SZ and Co-SZ eye drops could be raised from the different content of Zinc, which is involved in anti-oxidation.