荷人鼻咽癌裸鼠血内皮抑素的水平变化

Changes of plasma endostatin levels in nude mice bearing human nasopharyngeal carcinoma

目的 :探讨不同病期荷人鼻咽癌裸鼠血内皮抑素水平的变化 ,及其与肿瘤发展的关系。方法 :采用BALB/C裸小鼠复制荷人鼻咽癌裸鼠模型 ,通过EIA方法测定不同病期荷人鼻咽癌裸鼠血内皮抑素的浓度 ,称取瘤重。结果 :荷瘤鼠血中内皮抑素含量在种瘤 5d组 [(137 6 1± 5 3 4 1) μg/L]或 10d组 [(10 3 0 6± 17 33) μg/L]与正常裸鼠 [(113 5 6± 2 1 74 ) μg/L]比较未见明显差异 (P >0 0 5 ) ,而种瘤 2 0d组、30d组、4 0d组 [(2 12 80± 85 91) μg/L、(2 93 6 3± 6 2 5 3) μg/L、(2 71 5 7± 32 4 5 ) μg/L]显著高于正常裸鼠 (P <0 0 5 )。随着荷瘤时间的延长 ,瘤宿主血内皮抑素含量逐渐升高 ,种瘤 2 0d组明显增高 ,并持续维持在高水平。瘤重与血中内皮抑素含量呈正相关关系 (r=0 6 87)。结论 :提示肿瘤的发展与血内皮抑素含量的变化可能有关。

AIM: To approach the changes of endostatin levels in BALB/c nude mice bearing human nasopharyngeal carcinoma(NPC) in different period (5, 10, 20, 30 and 40 days) and the relationship between endostatin and tumor's development. METHODS: BALB/c nude mice bearing NPC was reproduced by hypodermic implantation of human CNE-2 cells into right-side of axillary fossa. The level of plasma endostation was detected, and the weight of isolated tumors was measured. On the basis of the regulation of these changes, their relationships were explored. RESULTS: At 5 days [(137.61±53.41) μg/L] or 10 days [(103.06±17.33) μg/L] endostatin level had no apparent alternation in comparison with control group [(113.56±21.74) μg/L, P>0.05]. At 20, 30 and 40 days concentration of endostatin[(212.80±85.91) μg/L,(293.63±62.53) μg/L, (271.57±32.45) μg/L, respectively] were higher than that of the control group (P<0.05). Along with the development of the tumors, both the levels of endostatin and tumors weight increased. There was a positive correlation between the level of endostatin and tumor weight (r=0.687, P<0.05). CONCLUSION:These results suggested that endostatin links with the development of NPC.