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基因芯片检测耐PHT点燃鼠脑突触可塑性基因的表达 被引量：1

Detection of the differential expression of genes with synapse plasticity in brains of the PHT-resistant/non-resistant epileptic rats with cDNA microarray
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摘要 目的:探索与鼠同源的人类已知基因在苯妥英钠耐药和非耐药癫痫鼠脑中的差异表达,了解难治性癫痫形成(PHT)机制。方法:建立耐药和非耐药组癫痫大鼠模型,成功后,处死动物,取出脑组织,常规抽提,逆转录生成PHTmRNAcDNA后,应用含有条人类基因的表达谱芯片,检测两者间基因表达谱的差异。4096cDNA结果:发现耐和治疗有效癫痫PHTPHT鼠与神经元突触可塑性有关的基因有条存在差异表达。18结论:突触可塑性增强可能是难治性癫痫形成的重要原因。 Objective: To determine the differential synapse plasticity-associated gene expression in PHT-resistant and PHT-responder epilepsy rat brain. Methods: Phenytoin-resistant and phenytoin-responder epilepsy rat model was build and the genes expression associated with synapse plasticity by cDNA microarray were studied. The four thousand and ninety-six target genes were arrayed onto glass slide. Both the mRNA from phenytoin-resistant and phenytoin-responder brains of kindling rats were reversal transcribed to cDNAs as hybridization probes which were labeled with either Cy3-dUTP or Cy5-dUTP at the same time. After hybridization with BioDoor4096 cDNA microarray, the results were analyzed by ImaGene3.0 software. Results: In the PHT- resistant and non-resistant epileptic rats, eighteen differential expressed genes which are associated with snapse plasticity were identified. Conclusion: The enhancement of synapse plasticity may be one of the important causes of drug-resistant epilepsy.
作者 文世全 王学峰 晏勇
机构地区 重庆医科大学临床学院神经内科
出处 《重庆医科大学学报》 CAS CSCD 2002年第3期276-279,共4页 Journal of Chongqing Medical University
关键词 基因芯片 难治性癫痫 突触可塑性 cDNA microarray Intractable epilepsy Synapse plasticity
分类号 R742.1 [医药卫生—神经病学与精神病学] Q78 [生物学—分子生物学]
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参考文献9

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同被引文献13

  • 1胡震,陈忠平.基因表达系列分析的进展及其在神经肿瘤领域中的应用[J].中国神经肿瘤杂志,2005,3(2):148-154. 被引量：1
  • 2徐静,吕炳建,张昊,陈俭,朱益民,谷雪梅,来茂德.电子基因表达谱分析平台的建立及其应用[J].浙江大学学报（工学版）,2006,40(2):186-191. 被引量：3
  • 3Wang YY, Smith P, Murphy M, et al. Global expression profiling in epileptogenesis :does it add to the confusion7 Brain Pathol,2010,20: 1-16.
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  • 5Saha S, Sparks AB, Rago C, et al. Using the transcriptome to annotate the genome. Nat Biotechno1,2002 ,20 :508-512.
  • 6Brenner S, Johnson M, Bridgham J, et al. Gene expression analysis by massively parallel signature sequencing (MPSS)on microbead arrays. Nat Biotechnol,2000,18:630-634.
  • 7Hendriksen H, Datson NA, Ghijsen WE, et al. Altered hippoeampal gene expression prior to the onset of spontaneous seizures in the rat post-status epilepticus model. Eur J Neurosci ,2001,14 : 1475-1484.
  • 8Elliott RC, Miles MF, Lowenstein DH. Lowenstein, Overlapping mi- croarray profiles of dentate gyrus gene expression during development- and epilepsy-associated neurogenesis and axon outgrowth. J Neurosci, 2003,23:2218-2227.
  • 9Potschka H, Krupp E, Ebert U, et al. Kindling-induced overexpression of Homer 1A and its functional implications for epileptogenesis. Eur J Neurosci ,2002,16:2157-2165.
  • 10Nygaard V, Hovig E. Options available for profiling small samples:a review of sample amplification technology when combined with mi- croarray profiling. Nucleic Acids Res,2006,34:996-1014.

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重庆医科大学学报
2002年 第3期

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