免疫印迹法检测信号蛋白的三种显示系统比较

Comparison of three develop systems for immunoblotting in detecting signal proteins

目的 :对比、分析用于检测信号蛋白的三种免疫印迹显示系统的敏感性。方法 :用不同的免疫印迹显示系统检测Jurkat细胞中ZAP 70分子。结果 :采用辣根过氧化物酶 (HRP) 增强发光显示系统较HRP 双底物系统及碱性磷酸酶 (AP) 双底物系统至少敏感 5 0倍。结论 :HRP 增强化学发光免疫印迹是一种较敏感的检测系统。

Objective:To compare and analyze the sensitivities of three displaying systems for immunoblotting in detecting signal proteins.Methods:The protein ZAP 70 extracted from Jurkat cells was detected by three different develop systems.Results:The horseradish peroxidase(HRP) enhanced chemiluminesce displaying system is more sensitive at least 50 times than HRP double substrate system and alkaline phosphatase(AP) double substrate system.Conclusions:The HRP enhanced chemiluminesce displaying system for immunoblotting is sensitive in detecting signal proteins.