急性化脓性中耳炎豚鼠中耳粘膜神经分布和形态改变

Alterations of middle ear mucosa nerve of the guinea pig with acute suppurative otitis media

目的 观察急性化脓性中耳炎对中耳粘膜神经分布和形态变化的影响 .方法 12只纯白红目豚鼠麻醉后 ,在无菌条件下于右侧耳注射 1× 10 8个·L- 1的金葡菌液 10 0 μL,左耳作正常对照 ,制成急性化脓性中耳炎模型 . 3d后利用免疫组化 ABC方法观察神经肽 PGP9.5在两组中耳粘膜的分布和形态变化 .结果 正常中耳粘膜内 ,PGP9.5阳性的神经纤维呈网状分布 ,主要沿粘膜下中 ,小血管长轴走行 ,在炎症作用下中耳粘膜充血、水肿、增厚并有灶性出血 ,PGP9.5阳性的神经纤维明显减少 .结论 神经系统和免疫系统存在联系 。

AIM To explore the changes in the neural distribution of the middle ear mucosa in experimental acute suppurative otitis media of guinea pigs. METHODS 12 healthy guinea pigs were employed in the experiment. After general anaesthesia, by injecting 1×10 6 Staphylococcosis aureus intothe middle ear cavities of right ears with the left ears serving as controls, the acute suppurative otitis media model was established. Then three days later, immunohistochemical ABC method was undertaken to explore the neutral distribution of PGP9.5 in the middle ear mucosa of the guinea pigs. RESULTS In the normal middle ear mucosa, the PGP9.5 positive fibers distributed as a net web and went along the longitude of the vessels. The presence of inflammation in middle ear induced congestion, oedema, haemorrhage, and the positive fibers of PGP9.5 decreased. CONCLUSION The results indicate that the change of peptide possibly plays an important role in the mechanisms of otitis media development, and there may be some relations between the system nerve and immunity.