大鼠面神经切断及修复对面神经核磷酸化的影响

Effects of facial nerve axotomy and repair on phosphorylation in facial nucleus of rats

目的 探讨面神经损伤及修复对面神经核蛋白磷酸化的影响 .方法 采用免疫组化染色法 ,观察成年大鼠面神经切断和即刻端端吻合后面神经核磷酸苏氨酸和磷酸酪氨酸的时程变化 .结果 面神经切断后 ,损伤侧面神经核于损伤早期即出现磷酸苏氨酸和磷酸酪氨酸免疫染色强度的明显增加 ,术后 14 d已基本降至正常水平 .磷酸苏氨酸免疫染色部位主要位于面神经核运动神经元 ,而磷酸酪氨酸免疫染色阳性细胞为小胶质细胞 .面神经切断后吻合组面神经核磷酸苏氨酸和磷酸酪氨酸免疫染色强度的变化趋势同面神经单纯切断组 .

AIM To study the effects of facial nerve axotomy and repair on phosphorylation in facial nucleus of rats. METHODS Changes in the time course of phosphothreonine and phosphotyrosine in the facial nucleus following facial nerve transection and immediate end to end anastomosis in adult rats were analyzed by using immunohistochemical staining. RESULTS Transection of facial nerve resulted in a striking increase in the intensity of phosphothreonine and phosphotyrosine immunostaining at early stage in facialnucleus ipsilateral to the operation, and the intensity of immunostaining dropped to normal 14 days after the operation. The phosphothreonine immunoreactivity was restricted to facial motoneurons, while phosphotyrosine immunoreactivity was restricted to microglia. The pattern of increase in the density of phosphothreonine and phosphotyrosine immunostaining following an immediate end to end anastomosis was similar to that of the transection group. CONCLUSION The changes of signal transduction in facial nucleus caused by facial nerve transection mainly occur at early stage of injury.