心脏重塑时基因表达谱改变及基因调节网络的初步研究

Pilot study on changes of gene expression profiles and interaction of genes in cardiac hypertrophy

近年发展起来的基因芯片 (DNAmicroarray)技术可以一次检测千百种基因的表达水平 ,具有传统基因表达检测方法无可比拟的高通量特点。由此得到的基因表达谱不仅可以提供基因间相互作用的信息 ,还可以此为线索进一步探索这些基因在细胞中的功能。我们利用基因芯片技术检测不同肥厚状态下心脏及心肌细胞的基因表达谱 ,研究与心肌肥厚相关的基因表达谱变化 ,并对获得的系列基因表达谱进行聚类分析 ,找出表达行为相似的基因群组 ,为研究多个基因之间的相互关系提供线索 。

Newly developed high-throughput technology, DNA mi croarray (cDNA or oligonucleot ide) is able to determine the expression pattern of thousands of genes simult aneously. Gene expression profiles, obtained with DNA microarray, will provide t he basis for understanding how genes work together to guide the functions of cel ls. In order to discover gene expression changes related to distinct cardiac hy pertrophy status, myocardial gene expression profilings from different cardiac h ypertrophy models were examined by cDNA microarray in our study. Those changed genes were clustered to several groups, in each of which genes acted in similar exp ression behavior. We intend to provide insight into the interaction of genes and explore the research strategy for the complex system.