雷公藤甲素微乳凝胶对CIA大鼠T细胞相关因子调节作用研究

Regulatory Effects of Triptolide Microemulsion Gel on T Cell Related Factors in CIA Rats

目的:观察雷公藤甲素微乳凝胶(TP-MBGs)外用对CIA大鼠T细胞相关因子表达水平的影响,以探究其治疗类风湿性关节炎的作用机制。方法:将56只CIA模型大鼠随机分成空白组、模型组、基质组、TP灌胃组、TP微乳高剂量组、TP-MBGs高、中、低剂量组,给药28 d后处理,采用ELISA法检测各组大鼠皮肤(CD1α、IgA)、血清(IFN-γ、IL-6、TNF-α、ColⅡ、IL-10)及滑膜细胞上清液(IL-1、PGE 2、TNF-α)中相关细胞因子的表达水平。结果:与空白对照组比较,模型组皮肤中CD1α、血清中IFN-γ、IL-6、TNF-α和滑膜细胞上清液中IL-1、PGE 2、TNF-α的表达水平明显升高;与模型组比较,TP-MBGs中剂量组血清中的TNF-α及IL-6、滑膜细胞上清液中的IL-1及TNF-α表达水平显著降低;TP-MBGs高剂量组皮肤中CD1α、血清中的TNF-α及IFN-γ、滑膜细胞上清液中的IL-1及TNF-α的表达水平显著降低。结论:雷公藤甲素微乳凝胶外用治疗类风湿性关节炎,可能通过调节皮肤免疫功能及抑制相关细胞因子的分泌,从而起到局部及全身治疗作用。

Objective:To observe the effects of triptolide microemulsion gel(TP-MBGs)on the expression of T cell related factors in CIA rats,and to explore the mechanism of its treatment of rheumatoid arthritis. Methods:A total of 56 CIA rats were randomly divided into a blank group,a model group,a matrix group,a TP intragastric administration group,a TP microemulsion high dose group,a TP-MBGs high,middle and low dose group.After 28 days of administration,the expression level of the rat skin of CD1α,IgA,the serum of IFN-γ,IL-6,TNF-α,ColⅡ,IL-10 and the synovial cell supernatant of IL-1,PGE 2,TNF-α were detected by ELISA. Results:Compared with the blank control group,the expression of CD1α in skin,IFN-γ,IL-6,TNF-α in serum and IL-1,PGE 2,TNF-α in synovial cell supernatant were significantly increased in the model group.Compared with the model group,the expression levels of TNF-α and IL-6 in serum,IL-1 and TNF-α in the supernatant of synovial cells in the middle dose TP-MBGs group were significantly decreased than those in the model group.The expression of CD1α in skin,TNF-α and IFN-γ in serum,IL-1 and TNF-α in synovial cell supernatant decreased significantly in high dose TP-MBGs group. Conclusion:Tripterygium wilfordii microemulsion gel for external treatment of rheumatoid arthritis may play a local and systemic role by regulating skin immune function and inhibiting the secretion of related cytokines.