介孔二氧化硅-壳聚糖复合粒子的合成

Synthesis of mesoporous silica-chitosan composite particles

以十六烷基三甲基溴化铵(CTAB)为模板,通过正硅酸乙酯(TEOS)的水解获得二氧化硅粒子,通过冻干、灼烧,制得介孔二氧化硅纳米粒子(MSNs);以巯丙基三甲氧基硅烷为巯基化的硅烷化试剂,制得巯基化的介孔二氧化硅纳米粒子(MSNs-SH);弱酸性条件下,以N-(3-二甲氨基丙基)-N′-乙基碳二亚胺盐酸盐(EDCI)为缩合剂,将巯基乙酸与壳聚糖结构中的氨基缩合,获得巯基化的壳聚糖(CS-SH);最后通过MSNs-SH与CS-SH巯基连接制得纳米介孔二氧化硅-壳聚糖复合粒子(MSNs-SS-CS),并实现了对阿霉素(DOX)、布地奈德2种药物的包载。实验通过FTIR、紫外可见分光光度计、荧光分光光度计、马尔文激光粒度仪等对粒子的结构、粒径、Zeta电位、载药等性能进行表征。

Silica particles were obtained by hydrolysis of tetraethyl orthosilicate(TEOS)with cetyltrimethylammonium bromide(CTAB)as a template,which were lyophilized and burned to obtain the silica nanoparticles(MSNs).Mercapto-modified mesoporous silica nanoparticles(MSNs-SH)were prepared using 3-merraptnpropylt rimethnxysilane as a silylating agent.Under weakly acidic conditions,thiolated chitosan(CS-SH)was obtained by condensation of thioglycolic acid with the amino group in chitosan,using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDCI)as condensing agent.Finally,MSNs-SS-CS was prepared by linking MSNs-SH and CS-SH.The nanoparticles were used for encapsulation of doxorubicin(DOX)and budesonide.In addition,the structure,particle size,Zeta potential and drug loading properties of the particles were analyzed by Fourier transform infrared spectroscopy(FTIR),UV-visible spectrophotometer,fluorescence spectrophotometer and Malvern laser particle size analyzer.