磷脂囊泡阵列的制备及其在生物分析领域中的应用

Preparation Methods for Phospholipid Vesicles Arrays and Their Applications in Biological Analysis

磷脂囊泡的组分与真实细胞十分接近,它不仅可作为人造细胞模型,模拟细胞的结构与功能,还在生物分析方面有着广泛应用。磷脂囊泡阵列是进行高通量膜蛋白筛选和生物分析的良好平台。本文详细综述了匀质小磷脂囊泡阵列、异质小磷脂囊泡阵列、巨型磷脂囊泡阵列的制备方法,以及这些阵列在抗生素检测、酶反应、膜蛋白与膜的相互作用等生物分析方面的应用,评价了现有制备方法的优缺点,并提出了本领域未来的发展方向。

Lipid vesicles, composed of cell membrane components, are commonly used as an artificial cell model to mimic cell structure and functions. They have also been widely used in biological analysis. Liposome array is a high throughput platform for protein screening and bio-sensing. This review focuses on the preparation methods of homogeneous small lipid vesicle array, heterogeneous small lipid vesicle array, and giant lipid vesicle array, as well as their applications in antibiotics detection, enzymatic reactions, interactions between membrane proteins and lipid membranes. Besides, the advantages and disadvantages of the reported methods are summarized and the future directions in this field are proposed.