腹腔致敏途径下的大鼠被动皮肤过敏试验影响因素研究

Study on Influential Factors of Passive Cutaneous Anaphylaxis Test in Rats with Abdominal Sensitization

目的研究腹腔注射给药致敏途径下,不同因素对大鼠被动皮肤过敏试验的影响。方法致敏原选用卵白蛋白,佐剂为弗氏完全佐剂,研究不同致敏剂量(5,10,20 mg/只)、不同致敏次数(3,5次)、不同致敏血清制备时间(第10,14天)等条件下,大鼠被动皮肤过敏试验的敏感性。结果卵白蛋白5,10,20 mg/只致敏剂量下,各组在原液、1:2注射点均出现蓝斑,且蓝斑直径大于5 mm,其中以10,20 mg/只剂量致敏3次,末次致敏后第10天制备血清的阳性效果较好。结论大鼠被动皮肤过敏试验,致敏途径选择腹腔注射时,推荐卵白蛋白剂量为10 mg/只,致敏3次,末次致敏后第10天制备致敏血清。

Objective To study the effects of different factors on passive skin allergy test in rats sensitized by intraperitoneal injection.Methods Using ovalbumin as allergen,Freund’s complete adjuvant was added,the sensitivity of passive skin allergy test in rats under different conditions including different sensitizing doses(5,10,20 mg per rat),different sensitization times(3,5 times)and different preparation time of sensitized serum(at the 10th,14th day)was studied.Results At the doses of 5,10 and 20 mg ovalbumin per rat,each group showed blue spots at the original solution and 1:2 mixture injection point,and the diameter of the blue spots was greater than 5 mm;the positive effect was better when the rats were sensitized for 3 times at the doses of 10 or 20 mg ovalbumin per rat and the sensitized serum was prepared on the 10th day after the last sensitization.Conclusion In the passive cutaneous anaphylaxis test in rats,the dosage of ovalbumin is recommended to be 10 mg per rat by intraperitoneal injection.The sensitized serum is recommended to be prepared on the 10th day after the last sensitization.