摘要
以蒜头果(Malania oleifera)为实验材料,基于转录组数据分析,采用RT-PCR方法克隆获得蒜头果3-酮酯酰-CoA还原基因,命名为MoKCR1,GenBank登录号为:KX421278。序列分析显示MoKCR1基因cDNA开放阅读框全长为963 bp,编码320个氨基酸,属于KCR家族。序列比对分析显示MoKCR1具有KCR蛋白所具有的NADH结合结构域[G(X)3GXG(X)3A(X)3A(X)2G]和裂解有关的关键结构域[Y(X)3K],蒜头果KCR与木薯(Manihot esculenta)KCR的蛋白序列同源性为82.81%。与已知超长链KCR蛋白进行系统进化分析显示MoKCR1与巨尾桉(Eucalyptus grandis)关系较近。荧光定量PCR分析表明MoKCR1在蒜头果果实膨大期的表达量最高。本研究为最终揭示蒜头果神经酸生物合成提供了研究基础。
The study used Malania oleifera as materials and cloned the full length of the 3-ketoacyl-CoA reductase(KCR)gene based on the transcriptome of Malania oleifera with the method of RT-PCR technology,which was named MoKCR1(GenBank ID:KX421278).The sequence analysis showed that the full-length cDNA of MoKCR1 gene was 963 bp,encoding 320 amino acids,and belonged to KCR family.The sequence alignment revealed that MoKCR1 possessed the NADH binding domain of KCR protein[G(X)3GXG(X)3A(X)3A(X)2G]and the key domain related to cleavage[Y(X)3K].The protein sequence homology of KCR in Malania oleifera and Manihot esculenta was 82.81%.Phylogenetic analysis of the known super-long chain KCR protein showed that MoKCR1 was closely related to Eucalyptus grandis.The fluorescent quantitative PCR indicated that the expression of MoKCR1 gene was the highest at the fruit enlargement stage of Malania oleifera.This study could provide a basis for revealing the biosynthesis of Malania oleifera.
作者
李云琴
陈中华
原晓龙
王毅
Li Yunqin;Chen Zhonghua;Yuan Xiaolong;Wang Yi(The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,The Key Laboratory of Forestry Plant Cultivation and Utilization,Yunnan Academy of Forestry,Kunming650201)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第14期4580-4585,共6页
Molecular Plant Breeding
基金
国家自然科学青年科学基金项目(31860177)
云南省应用基础研究计划青年项目(2017FD169)
云南省林业科学院创新基金项目(QN2018-01)共同资助
