黑糯米酒中总花色苷测定方法的研究

Determination method of total anthocyanin in black glutinous rice wine

以黑糯米酒为研究对象,对黑糯米酒花色苷的测定方法进行了研究。结果表明,pH示差法、单一pH法和差减法所测标准曲线的相关系数R2分别为0.997 7、0.998 5、0.999 4,线性关系均较好,均可用于测定黑糯米酒中总花色苷含量。3种方法测定黑糯米酒花色苷的平衡温度为30℃,平衡时间为40 min,测定波长为511 nm。单一pH法和差减法的最适pH值为0.8,pH示差法最适pH值为0.8和4.5。3种方法可直接测定黑糯米酒总花色苷含量,且精密度良好,其中pH示差法精密度最高,相对标准偏差(RSD)为0.83%。pH示差法加标回收率试验结果RSD为0.38%,表明该优化条件后的方法可靠、准确,适用于黑糯米酒中总花色苷测定。

Using black glutinous rice wine as research object,the determination method of anthocyanin in black glutinous rice wine was studied.The results showed that the correlation coefficient of the standard curve determined by pH differential method,single pH method and subtraction method were 0.997 7,0.998 5 and 0.999 4,respectively,and the linear relationship was all good,which could be used to determine the total anthocyanins content in black glutinous rice wine.The equilibrium temperature,time and measurement wavelength of three kinds of methods for the determination of anthocyanin in black glutinous rice wine was 30℃,40 min,and 511 nm,respectively.The optimum pH for the single pH method and the subtraction method was 0.8,and the optimum pH for the pH differential method was 0.8 and 4.5.Three kinds of methods could directly determine the total anthocyanins content in black glutinous rice wine,and the precision was good.Among them,the pH differential method had the highest precision,and the relative standard deviation(RSD)was 0.83%.The RSD of standard recovery rate test of the pH differential method was 0.38%,which indicated that the optimized method was reliable,accurate,and was suitable for the determination of total anthocyanins in black glutinous rice wine.